Fundamentals of Cell Immobilisation Biotechnology

Author: Viktor Nedovic,Ronnie Willaert

Publisher: Springer Science & Business Media

ISBN: 9401716382

Category: Science

Page: 555

View: 9936

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Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.

Immobilisierte Biokatalysatoren

Eine Einführung

Author: Winfried Hartmeier

Publisher: Springer-Verlag

ISBN: 3662078627

Category: Science

Page: 206

View: 7776

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Obwohl oder gerade weil eine kaum mehr überschaubare Zahl von Publi kationen auf dem Gebiet der Immobilisierung von Biokatalysatoren existiert, ist es für den Anfänger, der einen Einstieg sucht, auf diesem Gebiet oft schwer, geeignete Informationen aus der Flut von Originalarbeiten herauszufinden. Einige gute, fast immer aber eng lischsprachige Monographien sind entweder außerordentlich umfang reich und teuer, oder sie behandeln nur ausgewählte Teilaspekte der Immobilisierung. Dem will die vorliegende Einführung abhelfen. Sie soll dem Leser zu einem raschen Überblick verhelfen und ihm den inzwischen abgesicherten Kenntnisstand nach Art eines kurzen Lehr buches vermitteln. Das Buch ist aus Erfahrungen in Vorlesungen, Praktika und Kursen entstanden. Es soll sich maßgeblich an Lernende wenden, auch wenn "Immobilisierte Biokatalysatoren" bisher noch nicht zum Stan dardrepertoire der Lehre an deutschen Universitäten und Fachhoch schulen gehören. Es ist jedoch die Oberzeugung des Autors, daß sich dies in den kommenden Jahren mit der wachsenden Bedeutung dieses Gebietes und mit der allgemeinen Etablierung der Biotechnologie an den Hochschulen ändern wird. Ober den Kreis der Studenten und Dozen ten hinaus wendet sich die Einführung aber auch an die vielen Natur wissenschaftler und Techniker der industriellen Praxis, die sich mit immobilisierten Biokatalysatoren befassen und sich dazu ein ent sprechendes Grundwissen aneignen wollen.

Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Springer Science & Business Media

ISBN: 1597450537

Category: Science

Page: 449

View: 3610

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The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Enzyme Immobilization

Advances in Industry, Agriculture, Medicine, and the Environment

Author: Alka Dwevedi

Publisher: Springer

ISBN: 3319414186

Category: Science

Page: 132

View: 9953

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This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes

Analytical Applications of Immobilized Enzymes and Cells

Applied Biochemistry and Bioengineering

Author: Lemuel B. Wingard,Ephraim Katchalski-Katzir,Leon Goldstein

Publisher: Elsevier

ISBN: 1483215857

Category: Science

Page: 328

View: 9409

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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Environmentally Friendly Syntheses Using Ionic Liquids

Author: Jairton Dupont,Toshiyuki Itoh,Pedro Lozano,Sanjay Malhotra

Publisher: CRC Press

ISBN: 1466579765

Category: Science

Page: 160

View: 1729

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Increased environmental consciousness within the scientific community has spurred the search for environmentally friendly processes as alternatives to conventional organic solvents. In the past two decades, numerous advances—including the use of ionic liquids—have made it possible to develop substitutes for some toxic solvents. Ionic liquids are widely recognized as suitable for use in organic reactions and can also improve the control of product distribution, enhanced reactivity, ease of product recovery, catalyst immobilization, and recycling. Environmentally Friendly Syntheses Using Ionic Liquids presents the latest developments in the field. It also reviews the latest applications in a wide range of fields including biotechnology, nuclear science, medicine, pharmaceuticals, environmental science, and organic and inorganic chemistry—all from the standpoint of green sustainable chemistry. Growing interest in the field of ionic liquids will define newer and unexplored areas of applications, expanding possible use of these environmentally friendly chemicals. The information presented in this book will undoubtedly help motivate readers to further explore the field.

Microbial enzymes and biotransformations

Author: José-Luis Barredo

Publisher: Humana Pr Inc

ISBN: 9781588292537

Category: Medical

Page: 319

View: 1955

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The application of recombinant DNA technology to industrial fermentation and the production of enzymes over the last 20 years has produced a host of useful chemical and biochemical substances. In Microbial Enzymes and Biotransformations, leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Two overview chapters consider microorganisms as a source of metabolic and enzymatic diversity, and enzymes in the fast-moving field of enzyme biosensors. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Microbial Processes and Products, provides laboratory procedures for a wide range of processes and products mediated by microorganisms. Comprehensive and highly practical, Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Advances in Food Biotechnology

Author: Ravishankar Rai V

Publisher: John Wiley & Sons

ISBN: 1118864557

Category: Science

Page: 752

View: 6958

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The application of biotechnology in the food sciences has led to an increase in food production and enhanced the quality and safety of food. Food biotechnology is a dynamic field and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food. Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics: GMOs and food security issues Applications of enzymes in food processing Fermentation technology Functional food and nutraceuticals Valorization of food waste Detection and control of foodborne pathogens Emerging techniques in food processing Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research and regulatory agencies and those who are studying and teaching food biotechnology.

Molecular Biology and Biotechnology

Author: John M. Walker,Ralph Rapley

Publisher: Royal Society of Chemistry

ISBN: 0854041257

Category: Science

Page: 604

View: 1219

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This popular textbook has been revised and updated to provide a comprehensive overview and to reflect the latest developments in this rapidly developing area. Continuing with the broad base style of both current molecular and traditional biotechnology, chapters have been updated to reflect current interest and include new areas such as stem cell technology and important areas in drug discovery such as IP and patents. By presenting information in an easily assimilated form, this book makes an ideal undergraduate text for students of biology and chemistry, as well as to postgraduates.

Immunocytochemical Methods and Protocols

Author: Lorette C. Javois

Publisher: Springer Science & Business Media

ISBN: 1592595219

Category: Biomedicine

Page: 435

View: 8978

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Lorette Javois' timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: a useful, sometimes beautiful compilation of protocols and methods - FEBS Letters an extremely useful vade mecum' of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use.

Fundamentals of Food Biotechnology

Author: Byong H. Lee

Publisher: John Wiley & Sons

ISBN: 1118384911

Category: Science

Page: 544

View: 1985

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Food biotechnology is the application of modern biotechnologicaltechniques to the manufacture and processing of food, for examplethrough fermentation of food (which is the oldest biotechnologicalprocess) and food additives, as well as plant and animal cellcultures. New developments in fermentation and enzyme technologicalprocesses, molecular thermodynamics, genetic engineering, proteinengineering, metabolic engineering, bioengineering, and processesinvolving monoclonal antibodies, nanobiotechnology and quorumsensing have introduced exciting new dimensions to foodbiotechnology, a burgeoning field that transcends many scientificdisciplines. Fundamentals of Food Biotechnology, 2nd edition is basedon the author’s 25 years of experience teaching on a foodbiotechnology course at McGill University in Canada. The book willappeal to professional food scientists as well as graduate andadvanced undergraduate students by addressing the latest excitingfood biotechnology research in areas such as genetically modifiedfoods (GMOs), bioenergy, bioplastics, functionalfoods/nutraceuticals, nanobiotechnology, quorum sensing andquenching. In addition, cloning techniques for bacterial and yeastenzymes are included in a “New Trends and Tools”section and selected references, questions and answers appear atthe end of each chapter. This new edition has been comprehensively rewritten andrestructured to reflect the new technologies, products and trendsthat have emerged since the original book. Many new aspectshighlight the short and longer term commercial potential of foodbiotechnology.


Theorie und Methoden

Author: Hans Bisswanger

Publisher: Wiley-Blackwell

ISBN: 9783527300969

Category: Science

Page: 282

View: 7760

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Mit diesem Buch verfügen Biochemiker, Biologen und Mediziner - Studierende und Wissenschaftler - über eines der ausführlichsten Werke auf dem Gebiet der Enzymkinetik in aktualisierter und erweiterter Form. Der Text ist eine benutzerfreundliche, ausgewogene Mischung aus Theorie und Praxis. Alle wichtigen theoretischen Aspekte werden auch dem Einsteiger verständlich vermittelt. Dabei wird Wert darauf gelegt, daß stets der Bezug zum biologischen System sichtbar bleibt. Ein ausführlicher methodischer Teil gibt dem Praktiker im Labor eine wertvolle Unterstützung für die optimale Planung und Durchführung seiner Versuche. Methoden zur Bestimmung multipler Gleichgewichte, Kalorimetrie, die Messung schneller Reaktionen sowie elektrochemische und spektroskopische Methoden werden eingehend beschrieben. Das besondere Plus: die CD-ROM mit der weiterentwickelten und benutzerfreundlichen Version des zugehörigen Enzymkinetikprogramms.

Checkpoint Controls and Cancer

Author: Axel H. Schönthal

Publisher: Springer Science & Business Media

ISBN: 1592598110

Category: Medical

Page: 348

View: 6159

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Prestigious researchers working at the cutting-edges of their fields comprehensively review the complexities of checkpoint control model systems and provide experimental protocols to study the molecular components and their regulation. Volume 1 introduces all of the important components of checkpoint controls, describes their intricate interactions, and highlights the relevance of these processes to the cancer problem. Volume 2 provides techniques offering novel approaches, such as the use of genome databases and siRNA, and analyzes how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype.

Kirk-Othmer Chemical Technology and the Environment, 2 Volume Set

Author: Wiley

Publisher: John Wiley & Sons

ISBN: 0470105402

Category: Science

Page: 2573

View: 1041

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The two-volume reference work Chemical Technology and the Environment provides readers with knowledge on contemporary issues in environmental pollution, prevention and control, as well as regulatory, health and safety issues as related to chemical technology. It introduces and expands the knowledge on emerging "green" materials and processes and "greener" energy technology, as well as more general concepts and methodology including sustainable development and chemistry and green chemistry. Based on Wiley's renowned, Kirk-Othmer Encyclopedia of Chemical Technology, this compact reference features the same breadth and quality of coverage and clarity of presentation found in the original.

Molecular Biology and Biotechnology

A Comprehensive Desk Reference

Author: Robert A. Meyers

Publisher: John Wiley & Sons

ISBN: 9780471186342

Category: Medical

Page: 1034

View: 636

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Essential for the top of every desk and laboratory bench, this single volume reference is the first and last resource required to answer any question relating to molecular biology, biotechnology, or molecular medicine. This work contains the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. Over 250 carefully reviewed articles written by 378 eminent scientists in the field, provide detailed and readable coverage of today's most relevant topics including life processes at the molecular level, genetic disease diagnosis and genetic therapy, the theory and techniques for understanding, manipulating, and synthesizing biological molecules and their aggregates.

Methods in Non-Aqueous Enzymology

Author: Munishwar N. Gupta

Publisher: Springer Science & Business Media

ISBN: 9783764358037

Category: Science

Page: 218

View: 1308

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Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.

Bioconjugation Protocols

Strategies and Methods

Author: Christof M. Niemeyer

Publisher: Springer Science & Business Media

ISBN: 1592598137

Category: Medical

Page: 330

View: 9864

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Expert laboratorians update the classic bioconjugation methods and introduce valuable new techniques that go beyond pure biomedical applications to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanaoparticles, and carbon nanotube probes for atomic force microscopy.

Methods in Bioengineering

Systems Analysis of Biological Networks

Author: Arul Jayaraman,Juergen Hahn

Publisher: Artech House

ISBN: 1596934069

Category: Science

Page: 316

View: 2431

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"This cutting-edge volume provides a detailed look at the two main aspects of systems biology: the design of sophisticated experimental methods and the development of complex models to analyze the data. Focusing on methods that are being used to solve current problems in biomedical science and engineering, this comprehensive, richly illustrated resource shows you how to: design of state-of-the art methods for analyzing biological systems Implement experimental approaches for investigating cellular behavior in health and disease; use algorithms and modeling techniques for quantitatively describing biomedical problems; and integrate experimental and computational approaches for a more complete view of biological systems." --Book Jacket.

Thiol Redox Transitions in Cell Signaling

Author: N.A

Publisher: Academic Press

ISBN: 9780123810045

Category: Science

Page: 392

View: 3681

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This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines