Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Humana Press

ISBN: 9781493959884

Category: Science

Page: 377

View: 8202

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For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Immobilisierte Biokatalysatoren

Eine Einführung

Author: Winfried Hartmeier

Publisher: Springer-Verlag

ISBN: 3662078627

Category: Science

Page: 206

View: 3473

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Obwohl oder gerade weil eine kaum mehr überschaubare Zahl von Publi kationen auf dem Gebiet der Immobilisierung von Biokatalysatoren existiert, ist es für den Anfänger, der einen Einstieg sucht, auf diesem Gebiet oft schwer, geeignete Informationen aus der Flut von Originalarbeiten herauszufinden. Einige gute, fast immer aber eng lischsprachige Monographien sind entweder außerordentlich umfang reich und teuer, oder sie behandeln nur ausgewählte Teilaspekte der Immobilisierung. Dem will die vorliegende Einführung abhelfen. Sie soll dem Leser zu einem raschen Überblick verhelfen und ihm den inzwischen abgesicherten Kenntnisstand nach Art eines kurzen Lehr buches vermitteln. Das Buch ist aus Erfahrungen in Vorlesungen, Praktika und Kursen entstanden. Es soll sich maßgeblich an Lernende wenden, auch wenn "Immobilisierte Biokatalysatoren" bisher noch nicht zum Stan dardrepertoire der Lehre an deutschen Universitäten und Fachhoch schulen gehören. Es ist jedoch die Oberzeugung des Autors, daß sich dies in den kommenden Jahren mit der wachsenden Bedeutung dieses Gebietes und mit der allgemeinen Etablierung der Biotechnologie an den Hochschulen ändern wird. Ober den Kreis der Studenten und Dozen ten hinaus wendet sich die Einführung aber auch an die vielen Natur wissenschaftler und Techniker der industriellen Praxis, die sich mit immobilisierten Biokatalysatoren befassen und sich dazu ein ent sprechendes Grundwissen aneignen wollen.

Enzyme Immobilization

Advances in Industry, Agriculture, Medicine, and the Environment

Author: Alka Dwevedi

Publisher: Springer

ISBN: 3319414186

Category: Science

Page: 132

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This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes

Enzyme Biocatalysis

Principles and Applications

Author: Andrés Illanes

Publisher: Springer Science & Business Media

ISBN: 1402083610

Category: Technology & Engineering

Page: 392

View: 6499

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This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.


Analytical Applications of Immobilized Enzymes and Cells

Applied Biochemistry and Bioengineering

Author: Lemuel B. Wingard,Ephraim Katchalski-Katzir,Leon Goldstein

Publisher: Elsevier

ISBN: 1483215857

Category: Science

Page: 328

View: 8363

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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Environmentally Friendly Syntheses Using Ionic Liquids

Author: Jairton Dupont,Toshiyuki Itoh,Pedro Lozano,Sanjay Malhotra

Publisher: CRC Press

ISBN: 1466579765

Category: Science

Page: 160

View: 9714

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Increased environmental consciousness within the scientific community has spurred the search for environmentally friendly processes as alternatives to conventional organic solvents. In the past two decades, numerous advances—including the use of ionic liquids—have made it possible to develop substitutes for some toxic solvents. Ionic liquids are widely recognized as suitable for use in organic reactions and can also improve the control of product distribution, enhanced reactivity, ease of product recovery, catalyst immobilization, and recycling. Environmentally Friendly Syntheses Using Ionic Liquids presents the latest developments in the field. It also reviews the latest applications in a wide range of fields including biotechnology, nuclear science, medicine, pharmaceuticals, environmental science, and organic and inorganic chemistry—all from the standpoint of green sustainable chemistry. Growing interest in the field of ionic liquids will define newer and unexplored areas of applications, expanding possible use of these environmentally friendly chemicals. The information presented in this book will undoubtedly help motivate readers to further explore the field.

Fundamentals of Cell Immobilisation Biotechnology

Author: Viktor Nedovic,Ronnie Willaert

Publisher: Springer Science & Business Media

ISBN: 9401716382

Category: Science

Page: 555

View: 5016

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Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.

Enzymes Biotechnology Handbook

Author: NIIR Board of Consultants & Engineers

Publisher: ASIA PACIFIC BUSINESS PRESS Inc.

ISBN: 8178330784

Category: Biotechnology

Page: 490

View: 6279

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Industrial biotechnology is the practice of using cells to generate industrially useful products. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. Enzymes are the focal point of biotechnological processes, without them biotechnology as a subject would not exist. The main advantage of enzymes compared to most other catalysts is their stereo, region and chemo selectivity and specificity. Enzymes are responsible for many essential biochemical reactions in micro organisms, plants, animals, and human beings. Biotechnology processes may have potential in energy production, specifically in the substitution of renewable plant biomass for fossil feedstock. This will depend on the development of enzymes able to degrade cellulose in plant biomass and designing methods to recycle or dispose of spent biomass. With time, research, and improved protein engineering methods, many enzymes have been genetically modified to be more effective at the desired temperatures, pH, or under other manufacturing conditions typically inhibitory to enzyme activity (e.g. harsh chemicals), making them more suitable and efficient for industrial or home applications. Enzymes are used in the extraction of natural products, as catalysts in organic chemistry, in clinical analysis, in industrial processes, and so on. The application of enzymes is found in many different fields and it is one of the good sectors to venture. In coming few years it is estimated that world enzyme demand will average annual increases of 6.3 percent. This book basically deals with principles of industrial enzymology, basis of utilization of soluble and immobilized, enzymes in industrial processes, principles of immobilization of enzymes, enzymes in clinical analysis principles, practical aspects of large-scale protein purification, the applications of enzymes in industry, use of enzymes in the extraction of natural products, data on techniques of enzyme immobilization and bio affinity procedures etc. In this book you can find all the basic information required on the fundamental aspects of the enzymes, their chemistry, bio chemistry as well as detailed information of their applications a wide variety of industrial processes etc. The book is very useful for research scholars, technocrats, institutional libraries and entrepreneurs who want to enter into the field of manufacturing of enzymes.

Molecular Biology and Biotechnology

Author: John M. Walker,Ralph Rapley

Publisher: Royal Society of Chemistry

ISBN: 9780854046065

Category: Science

Page: 563

View: 7526

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This product is not available separately, it is only sold as part of a set. There are 750 products in the set and these are all sold as one entity. As a textbook, Molecular Biology and Biotechnology has always been immensely popular. Now in its fourth edition, it has been completely revised and updated to provide a comprehensive overview and to reflect all the latest developments in this rapidly expanding area. Written by recognised experts, the book aims to identify the impact that molecular biology has had on the development of biotechnology, with each of the nineteen chapters describing a specific subject area relevant to the subject. The impressive breadth of coverage includes areas such as plant biotechnology; food technology; vaccine development; the production of transgenic plants and animals; and the addition of an appropriate and timely new chapter devoted to bioinformatics. Presenting information in an easily assimilated form, Molecular Biology and Biotechnology makes an ideal undergraduate text. It will be of particular interest to students of biology and chemistry, as well as to scientists from outside the field requiring a rapid introduction to the subject.

Immunocytochemical Methods and Protocols

Author: Lorette C. Javois

Publisher: Springer Science & Business Media

ISBN: 1592595219

Category: Biomedicine

Page: 435

View: 6914

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Lorette Javois' timely new 2nd edition of Immunocytochemistry Methods revises and updates her widely acclaimed collection of step-by-step immunocytochemical methods, one that is now used in many biological and biomedical research programs. The methods are designed for researchers and clinicians who wish to visualize molecules in plant or animal embryos, tissue sections, cells, or organelles. In addition to cutting-edge protocols for purifying and preparing antibodies, light microscopic analysis, confocal microscopy, FACS, and electron microscopy, this revised edition contains many new methods for applying immunocytochemical techniques in the clinical laboratory and in combination with in situ hybridization. From Reviews of the First Edition: a useful, sometimes beautiful compilation of protocols and methods - FEBS Letters an extremely useful vade mecum' of comprehensive, detailed recipes, good advice and helpful information, spiral bound in a handy size for laboratory use.

Fundamentals of Food Biotechnology

Author: Byong H. Lee

Publisher: John Wiley & Sons

ISBN: 1118384911

Category: Science

Page: 544

View: 1214

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Food biotechnology is the application of modern biotechnologicaltechniques to the manufacture and processing of food, for examplethrough fermentation of food (which is the oldest biotechnologicalprocess) and food additives, as well as plant and animal cellcultures. New developments in fermentation and enzyme technologicalprocesses, molecular thermodynamics, genetic engineering, proteinengineering, metabolic engineering, bioengineering, and processesinvolving monoclonal antibodies, nanobiotechnology and quorumsensing have introduced exciting new dimensions to foodbiotechnology, a burgeoning field that transcends many scientificdisciplines. Fundamentals of Food Biotechnology, 2nd edition is basedon the author’s 25 years of experience teaching on a foodbiotechnology course at McGill University in Canada. The book willappeal to professional food scientists as well as graduate andadvanced undergraduate students by addressing the latest excitingfood biotechnology research in areas such as genetically modifiedfoods (GMOs), bioenergy, bioplastics, functionalfoods/nutraceuticals, nanobiotechnology, quorum sensing andquenching. In addition, cloning techniques for bacterial and yeastenzymes are included in a “New Trends and Tools”section and selected references, questions and answers appear atthe end of each chapter. This new edition has been comprehensively rewritten andrestructured to reflect the new technologies, products and trendsthat have emerged since the original book. Many new aspectshighlight the short and longer term commercial potential of foodbiotechnology.

Checkpoint Controls and Cancer

Author: Axel H. Schönthal

Publisher: Springer Science & Business Media

ISBN: 1592598110

Category: Medical

Page: 348

View: 7940

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Prestigious researchers working at the cutting-edges of their fields comprehensively review the complexities of checkpoint control model systems and provide experimental protocols to study the molecular components and their regulation. Volume 1 introduces all of the important components of checkpoint controls, describes their intricate interactions, and highlights the relevance of these processes to the cancer problem. Volume 2 provides techniques offering novel approaches, such as the use of genome databases and siRNA, and analyzes how cells of the human body can escape proper surveillance to grow into a tumor. Additional experimental methods are provided for the manipulation of checkpoint pathways and the analysis of the resulting consequences for the cellular phenotype.

Kirk-Othmer Chemical Technology and the Environment, 2 Volume Set

Author: Wiley

Publisher: John Wiley & Sons

ISBN: 0470105402

Category: Science

Page: 2573

View: 8997

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The two-volume reference work Chemical Technology and the Environment provides readers with knowledge on contemporary issues in environmental pollution, prevention and control, as well as regulatory, health and safety issues as related to chemical technology. It introduces and expands the knowledge on emerging "green" materials and processes and "greener" energy technology, as well as more general concepts and methodology including sustainable development and chemistry and green chemistry. Based on Wiley's renowned, Kirk-Othmer Encyclopedia of Chemical Technology, this compact reference features the same breadth and quality of coverage and clarity of presentation found in the original.

Molecular Biology and Biotechnology

A Comprehensive Desk Reference

Author: Robert A. Meyers

Publisher: John Wiley & Sons

ISBN: 9780471186342

Category: Medical

Page: 1034

View: 2549

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Essential for the top of every desk and laboratory bench, this single volume reference is the first and last resource required to answer any question relating to molecular biology, biotechnology, or molecular medicine. This work contains the theory and techniques for understanding, modifying, manipulating, expressing, and synthesizing biological molecules, conformations, and aggregates. Over 250 carefully reviewed articles written by 378 eminent scientists in the field, provide detailed and readable coverage of today's most relevant topics including life processes at the molecular level, genetic disease diagnosis and genetic therapy, the theory and techniques for understanding, manipulating, and synthesizing biological molecules and their aggregates.

Methods in Bioengineering

Systems Analysis of Biological Networks

Author: Arul Jayaraman,Juergen Hahn

Publisher: Artech House

ISBN: 1596934069

Category: Science

Page: 316

View: 1793

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"This cutting-edge volume provides a detailed look at the two main aspects of systems biology: the design of sophisticated experimental methods and the development of complex models to analyze the data. Focusing on methods that are being used to solve current problems in biomedical science and engineering, this comprehensive, richly illustrated resource shows you how to: design of state-of-the art methods for analyzing biological systems Implement experimental approaches for investigating cellular behavior in health and disease; use algorithms and modeling techniques for quantitatively describing biomedical problems; and integrate experimental and computational approaches for a more complete view of biological systems." --Book Jacket.

Thiol Redox Transitions in Cell Signaling

Author: N.A

Publisher: Academic Press

ISBN: 9780123810045

Category: Science

Page: 392

View: 5748

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This volume, along with its companion (volume 474), presents methods and protocols dealing with thiol oxidation-reduction reactions and their implications as they relate to cell signaling. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Over 450 volumes have been published to date, and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Along with companion volume, provides a full overview of techniques necessary to the study of thiol redox in relation to cell signaling Gathers tried and tested techniques from global labs, offering both new and tried-and-true methods Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Immobilized Cells

Principles and Applications

Author: J. Tampion,M. D. Tampion

Publisher: Cambridge University Press

ISBN: 9780521255561

Category: Medical

Page: 257

View: 5064

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This 1987 book gives a coherent overview of preparation and uses of immobilized enzymes.

Handbook of ELISPOT

Methods and Protocols

Author: Alexander E. Kalyuzhny

Publisher: Springer Science & Business Media

ISBN: 9781588294692

Category: Medical

Page: 321

View: 6338

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In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.

Surface Engineering of Polymer Membranes

Author: Zhi-Kang Xu,Xiao-Jun Huang,Ling-Shu Wan

Publisher: Springer Science & Business Media

ISBN: 9783540884132

Category: Technology & Engineering

Page: 348

View: 9317

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Surface Engineering of Polymer Membranes covers the processes that modify membrane surfaces to improve their in-service performance, meaning, to confer surface properties which are different from the bulk properties. Purposes may be to minimize fouling, modulate hydrophilicity/ hydrophobicity, enhance biocompatibility, create diffusion barriers, provide functionalities, mimic biomembranes, fabricate nanostructures, etc. First, the basics of surface engineering of polymer membranes are covered. Then topics such as surface modification by graft polymerization and macromolecule immobilization, biomimetic surfaces, enzyme immobilization, molecular recognition, and nanostructured surfaces are discussed. This book provides a unique synthesis of the knowledge of the role of surface chemistry and physics in membrane science. Dr. Zhikang Xu of the Institute of Polymer Science of Zhejiang University has eight Chinese patents and in 2006 was honored as a Distinguished Young Scholar by the National Natural Science Foundation of China (NNSFC).