Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Springer Science & Business Media

ISBN: 1597450537

Category: Science

Page: 449

View: 4552

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The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Immobilization of Enzymes and Cells

Author: Jose M. Guisan

Publisher: Springer Science & Business Media

ISBN: 1597450537

Category: Science

Page: 449

View: 9616

Release On

The continuing rapid progress in work designed to improve the functional properties of enzymes and cells as industrial catalysts has led to this revised, updated, and expanded new edition of the warmly received initial edition of Immobilization of Enzymes and Cells. This long-awaited second edition contains new and simplified protocols useful for industrial applications, novel techniques that will prove useful now or in the near future, and protocols for the preparation of immobilized derivatives suitable for a wide variety of nonconventional reaction media. The authors also offer tools for the development of new immobilization techniques, methods for preparing immobilized derivatives for therapeutic and industrial use, and new chemical reactors designed to overcome the limitations of immobilized derivatives. The emphasis is on improving enzyme and cell properties via very simple immobilization protocols, along with the development of new and better methods. The protocols follow the successful Methods in Biotechnology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Immobilization of Enzymes and Cells, Second Edition, affords biochemists, biotechnologists, and biochemical engineers a practical review of all the latest methods and tools-as well as optimized conventional techniques-needed to carry out successful research involving immobilizing enzymes and cells.

Biomedical Applications of Immobilized Enzymes and Proteins

Author: Thomas Ming Swi Chang

Publisher: Springer Science & Business Media

ISBN: 1468426109

Category: Technology & Engineering

Page: 448

View: 2686

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I) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N'E~ ~~ c) Soluble support 0 \:)....m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;..,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especially in the thera peutic area, is in a very early stage of development. Much remains to be explored and studied, and the area is wide open for investigators interested in original research in a new interdisciplinary area. References Chang, T. M. S., 1972, Artificial Cells, Charles C. Thomas, Publisher, Springfield, Ill. Dunlop, R. B. (ed.), 1974, Immobilized Biochemicals and Affinity ChrOTIULtography, Plenum Press, N ew York.

Analytical Applications of Immobilized Enzymes and Cells

Applied Biochemistry and Bioengineering

Author: Lemuel B. Wingard,Ephraim Katchalski-Katzir,Leon Goldstein

Publisher: Elsevier

ISBN: 1483215857

Category: Science

Page: 328

View: 2470

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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Molecular Biology of the Cell

Author: Bruce Alberts

Publisher: Garland Science

ISBN: 1317563743

Category: Science

Page: 1464

View: 4214

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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.


Enzyme Immobilization

Advances in Industry, Agriculture, Medicine, and the Environment

Author: Alka Dwevedi

Publisher: Springer

ISBN: 3319414186

Category: Science

Page: 132

View: 703

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This book covers the latest developments in enzyme immobilization with its wide applications, such as for industry, agriculture, medicine, and the environment. Topics covered include basics of enzyme immobilization, its implication in therapeutics and disease diagnostics, and its significance in solving environmental problems. This is an ideal book for researchers, graduate and postgraduate students, as well as scientists in industry, agriculture and health sectors. This book is a complete summary of enzyme immobilization and also thoroughly covers all the latest research. This book covers: The last one-hundred years of innovative research done in enzyme immobilization Recent developments in immobilization techniques, such as types of matrices, immobilization methods, and linking agents, as well as enzyme immobilization without any matrices and its properties The physiological and industrial significance of enzymes from plants and the implementation of immobilized enzymes in the treatment of waste water and polluted air Biomedical and bioanalytical applications of immobilized enzymes

Fundamentals of Cell Immobilisation Biotechnology

Author: Viktor Nedovic,Ronnie Willaert

Publisher: Springer Science & Business Media

ISBN: 9401716382

Category: Science

Page: 555

View: 7117

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Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.

Enzyme Technology

Author: Dr. S. Shanmugam

Publisher: I. K. International Pvt Ltd

ISBN: 9380026056

Category: Enzymes

Page: 232

View: 8931

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This book gives a broad account of enzymology and aim to put the current knowledge into perspective. The chapters follow a progression from the properties of isolated enzymes to the behaviour of enzymes in increasingly complex systems, leading up to the cell. Included is the discussion on the importance of enzymes in medicine and industry. This book discusses the behaviour of isolated enzymes, dealing in turn with isolation methods, structural characterization, kinetics, catalytic action and control of activity, immobilization methods and various applications of enzymes. The methods for isolation and characterization of enzymes are now well-established procedures, so the rate at which three-dimensional structures and mechanisms are being determined is increasing dramatically. Ultimately it is necessary to know the behaviour of enzymes in living cells. This involves in part a synthesis of the information obtained from the study of isolated enzymes, but it also requires detailed knowledge of the molecular morphology of the cell, which in turn requires methods for making measurements on intact cells. The study and application of enzymes have assumed increasing importance both in medicine and in industry and a discussion of these aspects is therefore given prime importance.

Techniques in Glycobiology

Author: R. Reid Townsend,Arland T. Hotchkiss

Publisher: CRC Press

ISBN: 9780824798222

Category: Science

Page: 648

View: 6766

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This work covers methodologies for plant and animal glycoconjugate analysis. It details mass spectrometry, nuclear magnetic resonance spectroscopy, glycolipids and new physical methods, o-glycosylation characterization, chromophore and fluorophore labelling of oligosaccharides, separations, exoglycosidases and mapping, and plant glycobiology.

Enzyme Nanoarchitectures: Enzymes Armored with Graphene

Author: N.A

Publisher: Academic Press

ISBN: 0128152419

Category: Science

Page: 419

View: 3499

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Enzymes Conjugated to Graphene, Volume 609 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Enzyme immobilization, Detection of Urea, Enzyme immobilization Enzyme immobilization, PAMAM dendrimer modified reduced graphene oxide post functionalized by horseradish peroxidase for biosensing H2O2, HRP immobilized for LEV detection, Enzyme immobilization, Graphene biocatalysts, Enzyme immobilization, Interactions, Enzyme immobilization, GQD, Enzyme Immobilization, and Enzyme immobilization on functionalized graphene oxide nanosheets. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods of Enzymology series Updated release includes the latest information on the enzymes conjugated to graphene

Enzyme and Microbial Biosensors

Techniques and Protocols

Author: Ashok Mulchandani

Publisher: Springer

ISBN: 1592594840

Category: Biosensors

Page: 264

View: 8315

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In Enzyme and Microbial Biosensors: Protocols and Techniques, master experimentalists detail cutting-edge methods developed in their own laboratories for the construction and evaluation of enzyme- and cell-based biosensors. The enzyme biosensors detailed here are based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, whereas the microbial biosensors are based on oxygen-gas electrode and optical techniques in which microorganisms are coupled to the signal transducer. All the techniques are thoroughly tested and are presented in sufficient detail to ensure robust and easily reproducible results. A companion volume, Affinity Biosensors: Protocols and Techniques, by Rogers & Mulchandani, concentrates on affinity biosensors based on optical, electrochemical, thermal, acoustic, and plasmon resource techniques as applied to nucleic acids, liposomes, and eukaryotic cells. Clearly the optimal starting point for all graduate students, postdoctoral and senior researchers, and technicians in academia, industry, and research establishments seeking rapid entry into the field of biosensors, Enzyme and Microbial Biosensors: Protocols and Techniques instantly becomes today's leading reference. Its step-by-step descriptions of various enzyme- and microbial-based biosensor techniques will allow both novices and experienced investigators alike successfully to apply these powerful new tools, and thus sharply enhance their laboratories' productivity.

Molecular Characterization of Autophagic Responses

Author: N.A

Publisher: Academic Press

ISBN: 0128097949

Category: Science

Page: 608

View: 2969

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Molecular Characterization of Autophagic Responses, Part B presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses Written in an accessible style by renowned experts in the field

Guide to Protein Purification

Author: Richard R. Burgess,Murray P. Deutscher

Publisher: Academic Press

ISBN: 9780123745361

Category: Medical

Page: 851

View: 843

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The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process.

DNA Repair Protocols

Mammalian Systems

Author: Daryl S. Henderson

Publisher: Springer Science & Business Media

ISBN: 1592599737

Category: Cytology

Page: 498

View: 4464

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In this second edition of a much praised laboratory manual devoted to eukaryotic systems, Daryl S. Henderson has refocused the book on mammalian cells, adding fourteen entirely new chapters and extensively revising many of the remaining chapters. The authors address a broad range of questions about practical mammalian DNA repair, including such arcana as "what is radioresistant DNA synthesis and how is it measured?" The techniques presented are readily reproducible and offer cutting-edge methods for cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of "adducted" bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, DNA Repair Protocols: Mammalian Systems, Second Edition, offers investigators a wide variety of productive methods to explore and make new discoveries in the world of mammalian DNA repair.

Methods for General and Molecular Microbiology

Author: C. A. Reddy,Terry J. Beveridge,John A. Breznak,George Marzluf

Publisher: American Society for Microbiology Press

ISBN: 1555812236

Category: Science

Page: 1069

View: 7500

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A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Microbial Enzymes and Biotransformations

Author: José-Luis Barredo

Publisher: Springer Science & Business Media

ISBN: 1592598463

Category: Medical

Page: 319

View: 2028

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Leading experts in enzyme manipulation describe in detail their cutting-edge techniques for the screening, evolution, production, immobilization, and application of enzymes. These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection. Overview chapters on microorganisms as a source of metabolic and enzymatic diversity, and on the fast-moving field of enzyme biosensors are presented. Microbial Enzymes and Biotransformations offers laboratory and industrial scientists a wealth of proven enzymatic protocols that show clearly how to go from laboratory results to successful industrial applications.

Protein Purification

Principles and Practice

Author: Robert K. Scopes

Publisher: Springer Science & Business Media

ISBN: 1475723334

Category: Science

Page: 380

View: 7448

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New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research that is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In so me cases the availability of texts in active research areas should help stimulate the creation of new courses.

Affinity Chromatography and Biological Recognition

Author: Irwin Chaiken

Publisher: Elsevier

ISBN: 0323146627

Category: Science

Page: 542

View: 6385

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Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.

Enzymes Biotechnology Handbook

Author: NIIR Board of Consultants & Engineers

Publisher: ASIA PACIFIC BUSINESS PRESS Inc.

ISBN: 8178330784

Category: Biotechnology

Page: 490

View: 2386

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Industrial biotechnology is the practice of using cells to generate industrially useful products. An enzyme is a protein that catalyzes, or speeds up, a chemical reaction. Enzymes are the focal point of biotechnological processes, without them biotechnology as a subject would not exist. The main advantage of enzymes compared to most other catalysts is their stereo, region and chemo selectivity and specificity. Enzymes are responsible for many essential biochemical reactions in micro organisms, plants, animals, and human beings. Biotechnology processes may have potential in energy production, specifically in the substitution of renewable plant biomass for fossil feedstock. This will depend on the development of enzymes able to degrade cellulose in plant biomass and designing methods to recycle or dispose of spent biomass. With time, research, and improved protein engineering methods, many enzymes have been genetically modified to be more effective at the desired temperatures, pH, or under other manufacturing conditions typically inhibitory to enzyme activity (e.g. harsh chemicals), making them more suitable and efficient for industrial or home applications. Enzymes are used in the extraction of natural products, as catalysts in organic chemistry, in clinical analysis, in industrial processes, and so on. The application of enzymes is found in many different fields and it is one of the good sectors to venture. In coming few years it is estimated that world enzyme demand will average annual increases of 6.3 percent. This book basically deals with principles of industrial enzymology, basis of utilization of soluble and immobilized, enzymes in industrial processes, principles of immobilization of enzymes, enzymes in clinical analysis principles, practical aspects of large-scale protein purification, the applications of enzymes in industry, use of enzymes in the extraction of natural products, data on techniques of enzyme immobilization and bio affinity procedures etc. In this book you can find all the basic information required on the fundamental aspects of the enzymes, their chemistry, bio chemistry as well as detailed information of their applications a wide variety of industrial processes etc. The book is very useful for research scholars, technocrats, institutional libraries and entrepreneurs who want to enter into the field of manufacturing of enzymes.